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Resveratrol Upregulated SIRT1, FOXO1, and Adiponectin and Downregulated PPARγ1–3 mRNA Expression in Human Visceral Adipocytes

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Abstract

Background

The SIRT1 enzyme is involved in adipose tissue (AT) lipolysis. FOXO1 is a protein that plays a significant role in regulating metabolism. Adiponectin is an adipokine, secreted by the AT, which has been considered to have an antiobesity function. PPARγ is one of the key actors in adipocytes differentiation. This study was undertaken to investigate whether resveratrol can regulate SIRT1, FOXO1, adiponectin, PPARγ1–3, and PPARβ/δ in human AT.

Methods

The effects of resveratrol were analyzed in freshly isolated adipocytes prepared from visceral fat tissue samples obtained during bariatric surgery. Genes messenger ribonucleic acid (mRNA) levels were determined by qRT-PCR.

Results

Ours results show that resveratrol modulates the studied genes, increasing SIRT1 (p = 0.021), FOXO1 (p = 0.001), and adiponectin (p = 0.025) mRNA expression and decreasing PPARγ1–3 (p = 0.003) mRNA in human visceral adipocytes.

Conclusions

Resveratrol, in vitro and at low concentration, modulates genes that are related to lipid metabolism, possibly preventing metabolic disease in human visceral adipose tissue (VAT).

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Abbreviations

AT:

Adipose tissue

VAT:

Visceral adipose tissue

FOXO1:

Forkhead/winged helix

SIRT1:

Sirtuin 1

PPAR:

Peroxisome proliferator-activated receptors

qRT-PCR:

Quantitative real-time polymerase chain reaction

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Acknowledgments

This study was supported by research grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Fundação de Apoio à Pesquisa do Rio Grande do Sul (FAPERGS). Dr. R. Margis is a recipient of a research fellowship from the CNPq.

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Authors and Affiliations

  1. Departamento de Bioquímica, ICBS, UFRGS, Rua Ramiro Barcelos 2600 Anexo, 90035-003, Porto Alegre, RS, Brazil

    Cíntia dos Santos Costa, Francieli Rohden, Thais Ortiz Hammes, Rogério Margis, Josiane Woutheres Bortolotto & Regina Maria Guaragna

  2. Centro de Biotecnologia, UFRGS, Av. Bento Gonçalves, Porto Alegre, RS, Brazil

    Rogério Margis

  3. Centro de Obesidade e Síndrome Metabólica, Hospital São Lucas da PUCRS, Porto Alegre, RS, Brazil

    Alexandre Vontobel Padoin & Cláudio Cora Mottin

Authors
  1. Cíntia dos Santos Costa
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  2. Francieli Rohden
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  3. Thais Ortiz Hammes
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  4. Rogério Margis
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  5. Josiane Woutheres Bortolotto
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  6. Alexandre Vontobel Padoin
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  7. Cláudio Cora Mottin
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  8. Regina Maria Guaragna
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Corresponding author

Correspondence to Regina Maria Guaragna.

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Costa, C.d.S., Rohden, F., Hammes, T.O. et al. Resveratrol Upregulated SIRT1, FOXO1, and Adiponectin and Downregulated PPARγ1–3 mRNA Expression in Human Visceral Adipocytes. OBES SURG 21, 356–361 (2011). https://doi.org/10.1007/s11695-010-0251-7

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  • DOI: https://doi.org/10.1007/s11695-010-0251-7

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