The effects of sodium hydrosulfide NaHS, a donor of hydrogen sulfide H2S, on the force of muscle contraction were examined on isolated myocardial strips from frog ventricles. NaHS decreased the amplitude of muscle contractions in a dose-dependent manner under normal conditions and during inhibition of Ca channels with nifedipine. In contrast, under conditions of blockade of ATP-dependent potassium channels with glibenclamide, NaHS exerted a positive inotropic effect from the first minute of application. Neither blockade, nor activation of ATP-dependent K-channels with glibenclamide modulated the negative inotropic effect of NaHS. Inhibition of K-channels with tetraethylammonium (TEA) (3, 5, 10 mM) or 4-aminopyridine increased the amplitude of myocardial contractions. Preliminary application of 4-aminopyridine or TEA (3 mM) did not eliminate NaHS-induced negative inotropic effect, although higher TEA concentrations (5 or 10 mM) prevented it. The data indicate that the targets of H2S in frog myocardium are ATP-dependent, Ca-activated, and voltage-dependent K-channels.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 151, No. 2, pp. 124-127, February 2011
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Sitdikova, G.F., Khaertdinov, N.N. & Zefirov, A.L. Role of Calcium and Potassium Channels in Effects of Hydrogen Sulfide on Frog Myocardial Contractility. Bull Exp Biol Med 151, 163–166 (2011). https://doi.org/10.1007/s10517-011-1280-5
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DOI: https://doi.org/10.1007/s10517-011-1280-5