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Effects of a protocol of ischemic postconditioning and/or captopril in hearts of normotensive and hypertensive rats

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Abstract

Brief periods (a few seconds) of cyclic coronary occlusions applied early in reperfusion induce a cardioprotection against infarct size, called postconditioning (PostC) in which B2-bradykinin receptors play a pivotal role. Since angiotensin-converting enzyme (ACE) inhibitors reduce degradation of kinins, we studied the effects of PostC on infarct size and postischemic myocardial dysfunction in both normotensive (WKY) and spontaneously hypertensive rats (SHR) acutely or chronically treated with the ACE inhibitor Captopril. Isolated hearts from SHR and WKY rats were subjected to the following protocols: (a) ischemia for 30- and 120-min reperfusion (I/R); (b) I/R + PostC protocol (5-cycles 10-s I/R); (c) pretreatment with Captopril for 4-weeks before to subject the hearts to I/R with or without PostC maneuvers. Some SHR hearts were treated with Captopril during the 20- or 40-min early reperfusion with or without PostC maneuvers. Cardiac function was assessed in vivo with echocardiography. Left ventricular pressure and infarct size were measured ex vivo. Chronic Captopril significantly reduced left ventricular hypertrophy in SHR, and reduced infarct size in both WKY and SHR hearts. PostC maneuvers significantly reduced infarct size in WKY, but not in SHR hearts. Yet, PostC slightly improved postischemic systolic function in untreated SHR. Captopril given in reperfusion was unable to limit I/R injury in SHR hearts. Data show that PostC protection against infarct size is blunted in SHR and that PostC is unable to add its protective effect to those of chronic Captopril, which per se reduces cardiac hypertrophy and heart susceptibility to I/R insult.

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Acknowledgments

The authors were supported by Compagnia di S. Paolo, National Institutes of Cardiovascular Research (INRC, FM, PP); Regione Piemonte (PP), PRIN (PP), ex-60% (CP, PP). The authors wish to thank Prof. Donatella Gattullo for insightful suggestions.

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  1. Dipartimento di Scienze Cliniche e Biologiche, dell’Università di Torino, Regione Gonzole 10, 10043, Orbassano (TO), Italy

    Claudia Penna, Francesca Tullio, Francesca Moro, Anna Folino, Annalisa Merlino & Pasquale Pagliaro

  2. Istituto Nazionale Ricerche Cardiovascolari, Bologna, Italy

    Claudia Penna, Francesca Moro, Anna Folino & Pasquale Pagliaro

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  1. Claudia Penna
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  2. Francesca Tullio
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  3. Francesca Moro
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  4. Anna Folino
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  6. Pasquale Pagliaro
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Correspondence to Pasquale Pagliaro.

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Penna, C., Tullio, F., Moro, F. et al. Effects of a protocol of ischemic postconditioning and/or captopril in hearts of normotensive and hypertensive rats. Basic Res Cardiol 105, 181–192 (2010). https://doi.org/10.1007/s00395-009-0075-6

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