Abstract
Biotin is known to influence hepatic glucokinase (GK) expression both at a transcriptional and at a translational level. The aim of the present paper was to investigate the effect of biotin on pancreatic GK. For this purpose, RIN 1046-38 cells were cultured in the presence of different biotin concentrations for different times; there-after, GK mRNA expression, GK activity and insulin release were studied. Results demonstrated that biotin has a biphasic effect on GK mRNA expression, being stimulatory after short-term treatment and inhibitory after long-term treatment. GK activity was increased after long-term treatment. Insulin release was not affected by biotin treatment. These data suggest that biotin may influence glucose metabolism also by acting directly at the level of beta-cells.
Article PDF
Similar content being viewed by others
References
Colowick SP, The hexokinases. In: Boyer PD (ed) The enzymes, Vol 9. Academic Press, New York, pp 1–48, 1973
Lawrence GM, Trayer IP, Hexokinase isoenzymes: antigenic cross-reactivities and aminoacid compositional relatedness. Comp Biochem Physiol [B] 7:233–238, 1984
Iynedjian PB, Mobius G, Seitz HJ, Wolheim CB, Renold AE, Tissue-specific expression of glucokinase: identification of the gene product in liver and pancreatic islets. Proc Natl Acad Sci USA 83:1998–2001, 1986
Bedoya FJ, Wilson JM, Gosh AK, Finegold D, Matchinsky FM, The glucokinase glucose sensor in human pancreatic islet tissue. Diabetes 35:61–67, 1986
Matchinsky FM, Glucokinase as glucose sensor and metabolic signal generator in pancreatic β cells and hepatocytes. Diabetes 39:647–652, 1990
Spence JT, Pitot HC, Hormonal regulation of glucokinase in primary cultures of rat hepatocytes. J Biol Chem 254:12331–12336, 1979
Minderop RH, Hoeppner W, Seitz HJ, Regulation of hepatic glucokinase gene expression. Role of carbohydrates, and glucocorticoid and thyroid hormones. Eur J Biochem 164:181–187, 1987
Iynedjian PB, Ucla C, Mach B, Molecular cloning of glucokinase cDNA. Developmental and dietary regulation of glucokinase mRNA in rat liver. J Biol Chem 262:6032–6038, 1987
Iynedjian PB, Gjinovci A, Renold AE, Stimulation by insulin of glucokinase gene transcription in liver of diabetic rats. J Biol Chem 263:740–744, 1988
Matschinsky FM, Ellerman JE, Metabolism of glucose in the islets of Langerhans. J Biol Chem 243:2730–2736, 1968
Bedoya FJ, Matschinsky FM, Shimizu T, O'Neil JJ, Appel MC, Differential regulation of glucokinase activity in pancreatic islets and liver of the rat. J Biol Chem 261:10760–10764, 1986
Magnuson MA, Shelton KD, An alternate promoter in the glucokinase gene is active in the pancreatic β cell. J Biol Chem 264:15936–15942, 1989
Iynedjian PB, Pilot PR, Nouspikel T, Milburn JL, Quaade C, Hughes S, Ucla C, Newgard CB, Differential expression and regulation of the glucokinase gene in liver and islets of Langerhans. Proc Natl Acad Sci USA 86:7838–7842, 1989
Dakshinamurti K, Chauhan J, Regulation of biotin enzyme. Annu Rev Nutr 9:211–233, 1988
Dakshinamurti K, Chauhan J, Biotin. Vitam Horm 45:337–388, 1989
Spence JT, Koudelka AP, Effects of biotin upon the intracellular level of cGMP and the activity of glucokinase in cultured rat hepatocytes. J Biol Chem 259:6393–6396, 1984
Chauhan J, Dakshinamurti K, Transcriptional regulation of the glucokinase gene by biotin in starved rats. J Biol Chem 266: 10035–10038, 1991
Clark SA, Burnham BL, Chick WL, Modulation of glucose-induced insulin secretion from a rat clonal β cell line. Endocrinology 127:2779–2788, 1990
Danielson PE, Fors-Petter S, Brow MA, Calavetta L, Douglas J, Milner R, Sutcliffe G, p1B15: a cDNA clone of the rat mRNA encoding cyclophilin. DNA 7:261–267, 1988
Chamberlain JS, Gibbs RA, Ranier JE, Caskey CT, Multiplex PCR for the diagnosis of Duchenne muscular distrophy. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, CA, pp 272–281, 1990
Herbert V, Gottlieb CW, Bleicher SJ, Coated charcoal immunoassay of insulin. J Clin Endocrinol Metab 25:1375–1384, 1965
Mooradian AD, Morley JE, Micronutrient status in diabetes mellitus. Am J Clin Nutr 45:877–895, 1987
Coggeshall JC, Heggers JP, Robson MC, Baker H, Biotin status and plasma glucose in diabetics. Ann NY Acad Sci 447:389–392, 1985
Singh IN, Dakshinamurti K, Stimulation of guanylate cyclase and RNA polymerase II activities in HeLa cells and fibroblasts by biotin. Mol Cell Biochem 79:47–55, 1988
Vesely DL, Biotin enhances guanylate cyclase activity. Science 216:1329–1330, 1982
Fernandez-Mejia C, Davidson MB, Regulation of glucokinase and proinsulin gene expression and insulin secretion in RIN-m5F cells by dexamethasone, retinoic acid and thyroid hormone. Endocrinology 130:1660–1668, 1991
Hughes SD, Qaade C, Milburne JL, Cassidy L, Newgard CB, Expression of normal and novel glucokinase mRNA in anterior pituitary and islet cells. J Biol Chem 266:4521–4530, 1991
Reddi A, De Angelis B, Frank O, Lasker N, Baker H, Biotin supplementation improves glucose and insulin tolerance in genetically diabetic kk mice. Life Sci 42:1323–1330, 1988
Maebashi M, Makino Y, Furukawa Y, Ohinata K, Kimura S, Sato T, Therapeutic evaluation of the effect of biotin on hyperglycemia in patients with non-insulin dependent diabetes mellitus. J Clin Biochem Nutr 14:211–218, 1993
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Borboni, P., Magnaterra, R., Rabini, R.A. et al. Effect of biotin on glucokinase activity, mRNA expression and insulin release in cultured beta-cells. Acta Diabetol 33, 154–158 (1996). https://doi.org/10.1007/BF00569427
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00569427