Blood samples
| Routine clinical laboratory sampling | Full blood count, urea and electrolytes, bone profile, liver function tests, glucose, HBA1c, lipid profile, ferritin, B12, folate, coagulation screen, CRP |
| Additional clinical laboratory sampling | NTpro-BNP, D Dimer, hsTnI, renin, aldosterone, RAS fingerprinting |
| Urine sampling | 24-hour urine collection for electrolytes (optional) |
| Vascular phenotyping | 24-hour ABPM, ECG, brachial flow-mediated dilation (FMD), HBPM |
| SARS-CoV-2 antibody test | IgG antibody testing |
| Vascular and immune biomarkers (may be stored) | Vascular biomarkers Microparticles, oxidative stress markers (TBARS, 8-OHDG, nitrotyrosine), total antioxidant status, inflammatory biomarkers/adhesion molecules (ILs, MCP1, TNF, IFNs, sVCAM-1, sICAM, s-Selectin), RAS fingerprint ACE2 (s-ACE2, ACE2 activity), Ang peptides Immune biomarkers Trucount panel (CD3, CD4, CD8, CD14, CD19, CD45, CD56, HLA-DR), T cells (CD3, CD4, CD8, CD16, CD27, CD45RA, CD56, CD62L, TcRα/β TcRγ/δ), T cells II (CD3, CD4, CD8, CD31, CXCR4, CD27, CD45RA, CD56, CD40L), T cells III (CD3, CD4, CD45RA, CXCR5, CXCR, CCR6, CCR7, ICOS, PD-1), regulatory T cells (CD3, CD4, CD8, TcRα/β, CD25, CD45RA, CD127, FoxP3 (i/c)), B cells (CD5, CD10, CD19, CD24, CD27, CD38, CD86, IgD, IgM), Monocytes (CD4, CD14, CD16, CD19, CD45, HLA-DR), Dendritic cells (CD1c, CD16, CD11c,CD3/CD14/CD15/CD56, CD123, CD141, HLA-DR, CD80, SLAN) Future biomarker studies Blood and urine to be stored for future ethically approved biomarker studies. |
ABPM, ambulatory blood pressure monitoring; CRP, C reactive protein; HBA1c, glycated hemoglobin; HBPM, home blood pressure monitoring; NTpro-BNP, N-terminal pro B-type natriuretic peptide; RAS, renin–angiotensin system.