Abstract
With an assay that generates free radicals (FR) through photooxidation of dianisidine sensitized by riboflavin, 4 x 10(-5) M captopril, epicaptopril (SQ 14,534, captopril's stereoisomer), zofenopril, and fentiapril [all sulfhydryl (-SH)-containing angiotensin-converting enzyme (ACE) inhibitors] were shown effective scavengers of nonsuperoxide free radicals whereas non-SH ACE inhibitors were not. Captopril was a more effective FR scavenger at pH 5.0 than at pH 7.5. Captopril (2 x 10(-5) M) also scavenged the other toxic oxygen species hydrogen peroxide and singlet oxygen and inhibited microsomal lipid peroxidation. Finally, captopril reduced the amount of superoxide anion-radical detected after neutrophils in whole blood were activated with zymosan, probably by inhibiting leukocyte superoxide production.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Angiotensin-Converting Enzyme Inhibitors / pharmacology*
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Animals
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Antioxidants / pharmacology*
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Captopril / pharmacology
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Dianisidine / pharmacology
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Drug Interactions
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Free Radical Scavengers
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Free Radicals / metabolism
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Granulocytes / drug effects
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Granulocytes / metabolism
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Lipid Peroxidation / drug effects*
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Male
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Microsomes, Liver / metabolism
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Neutrophils / drug effects
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Neutrophils / metabolism
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Oxidation-Reduction
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Oxygen / metabolism
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Oxygen Consumption / drug effects
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Photochemistry
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Rats
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Rats, Inbred Strains
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Stimulation, Chemical
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Sulfhydryl Compounds / pharmacology*
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Superoxides / metabolism
Substances
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Angiotensin-Converting Enzyme Inhibitors
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Antioxidants
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Free Radical Scavengers
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Free Radicals
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Sulfhydryl Compounds
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Superoxides
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Captopril
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Dianisidine
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Oxygen