RT Journal Article
SR Electronic
T1 MicroRNA 8059 as a marker for the presence and extent of coronary artery calcification
JF Open Heart
JO Open Heart
FD British Cardiovascular Society
SP e000678
DO 10.1136/openhrt-2017-000678
VO 5
IS 1
A1 Philippa Howlett
A1 Jane K Cleal
A1 Huihai Wu
A1 Nikunj Shah
A1 Alex Horton
A1 Nick Curzen
A1 Michael Mahmoudi
YR 2018
UL http://openheart.bmj.com/content/5/1/e000678.abstract
AB Objective MicroRNAs (miRNAs) may serve as potential biomarkers in a variety of pathologies. The aim of this study was to determine whether miRNAs could serve as blood-based markers of isolated coronary artery calcification (CAC) defined as CAC in the absence of an underlying metabolic abnormality.Methods 24 age-matched and sex-matched patients who had been referred for elective CT coronary calcium score and angiography as part of investigation for cardiac chest pain were recruited. Peripheral venesection was performed and an Agatston calcium score was derived from the CT coronary angiogram using default software. RNA was extracted using the LeukoLOCK Total RNA Isolation System for Toray’s microarray analysis and quantitative reverse transcription PCR (qRT-PCR).Results The patients were well matched for age, sex and conventional risk factors for coronary artery disease. Microarray analysis identified lower expression of miRNA-138-2-3p, miRNA-1181, miRNA-6816-3p and miRNA-8059 in patients with coronary artery calcium score (CACS)=0 vs CACS>100. qRT-PCR confirmed significant downregulation of miRNA-8059 in patients with CACS>100 (CACS=0 vs CACS>100; P=0.03).Conclusion miRNA-8059 may serve as a peripheral blood-based biomarker for the presence of CAC, as well as provide a platform for studying the pathophysiological basis of isolated CAC.Trial registration number NCT01992848; Results.